In the present study antibacterial activity of the plant extract i.e. Bombex ceiba Linn. (Bark & Fruit), Berberis spp. (Root) and Urtica diocia Linn. (Leaves) has been standardized for the ethno-botanical importance and to investigate the study of different plant parts extracted in different solvent system. The total five solvent systems were used for the extraction. The plant parts has been evaluated for their antimicrobial activity against Staphylococcus aureus MTCC- 96, Escherichia coli MTCC- 1610, Salmonella abony MTCC- 890, Micrococcus luteus MTCC-435, Pseudomonas spp., Lactobacillus plantarum, and against some fungal culture i.e. Aspergillus niger MTCC-2196, A. fumigatus MTCC-30, Penicillium citrinum and Candida albicans MTCC-1637.The results were found satisfactory against bacterial strain as compared to fungal strain in primary screening. .Further study MIC and MBC was evaluated against only bacterial stain. Antibacterial activity was performed by agar well diffusion method and denoted by inhibition zone diameter. Results were found very satisfactory. Almost all plant showed antibacterial activity against test organisms. These plant parts are useful in the remedy of ethnic group as well as aurvedic and traditional practioner for the treatment of various diseases.