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PLANTICA – Journal of Plant Science (ISSN: 2456 – 9259) Published By: Association of Plant Science Researchers – www.jpsr.in

PLANTICA 1(2), October, 2017

PLANTICA, Vol. 1 (2), October, 2017

Cover and Index

1.  A REVIEW ON SCREENING OF EXTRACELLULAR HYDROLYTIC ENZYME      PRODUCING MICROORGANISMS

Arun Kumar Sharma, Vaishali Srivastava and Vinay Sharma*

Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India *Corresponding Author: vinaysharma30@yahoo.co.uk 

Abstract:

In past few years the novel potential of utilizing microbes as biotechnological sources of enzymes of industrially important has encouraged improved attention in the investigation of extracellular enzymatic activity in numerous microbes. Commercial enzymes are generally extracted from microorganisms because of their biochemical and physiological properties, easy cultivation at large scale and easy genetic manipulation for improved productivity. Starch degrading enzymes such as amylase have received huge consideration due to their noticeable technological importance and economic advantages. Celluloses are observed as the most important renewable resource for bioconversion and cellulose is usually hydrolyzed cellulase enzyme. Proteases are the imperative industrial enzymes and cover about 25% of global commercial enzymes. Lipases are utilized in the modification of oils and fats, detergents, food processing, synthesis of pharmaceuticals and fine chemicals, manufacturing of paper and synthesis of pharmaceuticals and cosmetics, brewing, bakery, biofuels and processing of leather. These extracellular enzymes are manufactured by numerous microorganisms, usually by bacteria and fungi. Therefore present review deals with the screening of hydrolytic enzyme producers.

Key words: Amylase, cellulase, extracellular enzymes, lipase, microorganisms, protease.

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2. EFFECT OF MICRONUTRIENTS ON DIFFERENT YIELD PARAMETERS OF     SUGARCANE

Harsh Vardhan Chauhan*, Rekha Balodi and R.K. Sahu

Centre of Advance Faculty Training in Plant Pathology

G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand – 263145, India *Corresponding author: harshv136@gmail.com

Abstract:

Micronutrients are those nutrient elements, which are required in very small quantities for plant growth and development. The micronutrients essential for green plants are iron, copper, manganese, zinc, boron, molybdenum and chloride. Most of the micronutrients are important constituents of enzymes or co-enzymes produced by an organism for performing various physiological processes. When the availability of these elements is very low, it produces characteristic deficiency symptoms and the plant growth is affected. On the other hand, excess availability and uptake of these elements may cause phytotoxic symptoms resulting in lower yields. Hence, it is always essential to maintain their availability at optimum levels and in correct proportions for realizing the highest productivity. Micronutrients play a very vital role for growth and development of sugarcane. Micronutrients reduce the severity of disease. The results indicate that there was no disease incidence in Zinc sulphate, Borax, Zinc sulphate+Borax, Zinc sulphate+Copper sulphate+Borax and Borax+Elemental sulphur. Zn (zinc sulphate) + Cu (copper sulphate) + B (borax) not only reduced the disease but also enhance the yield and growth parameters i.e. clumps, tillers, cane girth, cane height, cane weight, nodes, internodes, brix, sucrops % juice purity, CCS (commercial cane sugar), cane yield and CCS yield. A very high amount of biomass is produced by sugarcane, which results in greater removal of micronutrients.

Key Words: Micronutrients, sugarcane and yield

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3. EFFECT OF NITROUS ACID TREATMENT ON LIPASE PRODUCTION BY          LOCAL SOIL FUNGAL ISOLATE

Arun Kumar Sharma, Sapna Kumari and Vinay Sharma*

Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India *Corresponding Author: vinaysharma30@yahoo.co.uk

Abstract:

The present investigation explains identification and strain improvement of Cunninghamella sp. for enhanced production of lipase in submerged fermentation. Four hyperproducer fungal isolates previously isolated from 4 different soil samples were identified belonging to genera Rhizopus, Cunninghamella and Aspergillus luchuensis by looking for macroscopic (Agar plate culture) and microscopic features (lactophenol cotton blue staining). Lipolytic potential of Cunninghamella sp. was increased by strain improvement using random (induced) mutagenesis technique with chemical mutagen (nitrous acid). Lipase activity of Cunninghamella sp. was increased up to 11 % (156.79 ± 3.19 U/ml) as compared to wild strain (141.23 ± 1.73 U/ml) after 90 minutes incubation with nitrous acid. This hyperproducer mutagenic strain was assigned the code name Cunninghamella sp. NA90. Lipase activity was decreased after short time exposure with nitrous acid whereas long time treatment increased lipase activity. Nitrous acid was found potent mutagenic agent for enhancing lipolytic potential of Cunninghamella sp.

Keywords: Lipase, strain improvement, Cunninghamella sp., hyperproducer, nitrous acid.

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4. EFFICACY OF ORGANIC FERTILIZER- AMIRTHAKARAISAL ON THE               GROWTH OF CHILLI PLANT

Anjali, Tanveer Hassan, Muralitharan R, Murugalatha N.*, Rinkey Arya,Vijay Kumar and Naveen Chandra

Department of Agriculture, Quantum School of Graduate Studies,Quantum Global Campus, Roorkee, Uttarakhand – 247667, India *Corresponding authornavikal123@gmail.com

Abstract:

Chilli plant belonging to Solonaceae family is one of the major cash crop grown in India. Chilli being a good source of vitamin is used in wide range of medicines against tonsillitis, diphtheria, loss of appetite etc., Application of inorganic fertiliser in soil for the growth of plants has deteriorated the quality of soil and reduced the plant growth and yield. The paper examines the effect of Amirthakaraisal, an organic fertiliser on the growth of chilli plant. The organic fertiliser Amirthakaraisal is prepared by using only the cow ingredients. The seeds of chilli plant were soaked in various concentration of organic manure (1%, 3%, 5% & 7%) prior germination. Organic fertiliser at the rate of 1, 3, 5 , 7 % concentration was sprayed on all plants. Plant height and root length were measured. Maximum shoot length and root length were observed in the plants treated with 7% organic manure. Effective results were obtained after application of organic fertilisers as compared to water as control.

Key Words: Chilli plant, organic fertiliser- Amirthakaraisal, soil fertility, growth

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5. PARTIAL PURIFICATION OF CUNNINGHAMELLA LIPASE

Arun Kumar Sharma, Sapna Kumari and Vinay Sharma*

Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India *Corresponding Author: vinaysharma30@yahoo.co.uk

Abstract:

Lipases are considered only after carbohydrases and proteases in global enzyme market and contribute to nearly 5% of enzyme market. They are found in animals, plants and microbes hence classified as animal, plant and microbial lipases. In most of the industries, they prefer exploitation of crude enzyme instead of purified preparation to reduce the cost and time of purification. Hence, the present study is focused on salt (ammonium sulfate) purification of extracellular lipase obtained from submerged fermentation of lipolytic Cunninghamella sp. Among all precipitates, highest level of lipase activity (101.18 ± 4.3 U/ml) and specific activity (7.36 ± 1.4 U/mg) was observed in 70% precipitate which suggest that lipase was maximally precipitated at 70% ammonium sulfate concentration. Hence, 70% salt concentration was found optimum for maximum precipitation of Cunninghamella lipase.

Keywords: Cunninghamella, ammonium sulfate, animal, lipase, precipitates, specific activity.

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6. RESPONSE OF PACLOBUTRAZOL AS SPRAY & DRENCH ON POT GROWN FUCHSIA CV. ‘PINK GALORE’

Yachna Gupta and Pooja Kaintura*

Department of Floriculture and Landscaping, Dr. Y. S. Parmar University of Horticulture & Forestry, Solan (H.P.), India *Corresponding author: poojakaintura29005@gmail.com

Abstract:

Present investigations were carried out at the experimental farm of Department of Floriculture and Landscaping, Dr. Y. S. Parmar University of Horticulture & Forestry, Solan (HP) with the objective to study the effect of paclobutrazol as drench, spray or combination of both on growth and flowering of Fuchsia cv. ‘Pink Galore’. The experiment was laid out in a completely randomized design consisting of 16 treatments, replicated thrice and each having 5 pots. When the cuttings were 8 to 10 cm in length, drench treatments of PP333 @ 10, 20 & 30 ppm were given per pot. After 30 days of drenching, PP333 @ 10, 20 & 30 ppm was applied as foliar spray. All the paclobutrazol treated plants resulted in reduced plant height, plant spread, internodal length, flower stalk length and flower size.Days to flower bud formation and flowering were advanced in drench and combination treatments, whereas, spray treatments delayed flower bud formation and flowering. The number of flowers open at a time and duration of flowering were increased in all paclobutrazol treated plants. The presentability of plants increased in all the paclobutrazol treated plants as compared to control. However, the most presentable pots were obtained when the plants were drenched with 10 ppm paclobutrazol.

Key words: Paclobutrazol (PP333), Fuchsia, presentability

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7. ISOLATION AND CHARACTERIZATION OF DESIRABLE COLOUR MUTANT    OF CARNATION

Pooja Kaintura

Department of Horticulture, VCSG Uttarakhand University of Horticulture and Forestry, Bharsar , Uttarakhand, 263145 *Corresponding author: poojakaintura29005@gmail.com

Abstract:

Development of new cultivars through conventional or modern techniques has been a great demand in commercial floriculture. New colour, earliness, stem length, number of flowers, plant architecture, resistance to abiotic and biotic stress, productivity and vase life are the main attributes required in new cultivars. Mutation breeding is an established method for crop improvement and has played a major role in the development of many new flower colour/shape mutant varieties in ornamentals. A spontaneous mutant was observed in a carnation at Floriculture Center of VCSG Uttarakhand University of Horticulture and Forestry, Bharsar, Uttarakhand during 2013.The mother plant of the mutant was variety ‘Tempo’. Initially plant showed sectoral chimera on flower but latter on mutant plant developed flower having change in whole colour of flower from creamish white to deep pink on few branches.

Key words: Carnation, mutant and isolation

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8. EFFECTS OF SALINITY STRESS CONDITION IN SEED GERMINATION            AND VIGOUR OF Aegle marmelos

Rakesh Singh, V.P Nautiyal, J.S. Chauhan and Ganga Dutt

Department of Seed Science and Technology, Hemwati Nandan Bahuguna Garhwal University (Central University), Srinagar, Uttarakhand, India, Corresponding author: negirakesh656@gmail.com

Abstract:

In cultivation of medicinal trees, stress conditions are very important problem. Salinity stress is major problem of increasing production in crop growing areas throughout the world. The objective of this research was to evaluate effect of salinity stress (NaCl) on seed germination and vigour of   bael (Aegle marmelos). The present study was conducted to examine the   effects of  Salinity stress (seeds were soaked in solution with concentration of 5% 10% and 15% NaCl for 3 hours)  treatment along with control (Without any treatment)  on seed germination and seedling quality character of bael (Aegle marmelos).  The results showed that the effect of salinity was significantly decrease seed germination percentage; seedling length, seedling vigour and dry matter production than control. Mean comparison showed that control and stress conditions treatments those after 25 days and the maximum germination (76.67), seedling length (14.4 cm), dry matter production (0.19gm), vigour index -I ( 1073.79), and vigour index 2 (14.22).were achieved in control (Without any treatment)  of bael seeds but in stress condition failed to improve germination. Hence bael seeds can be showed lower germiniability and vigour on Salinity stress condition.

Key words:  Salinity stress (NaCl), germination, seedling, vigour and bael.

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